A highly efficient method for washing mammalian spermatozoa
نویسندگان
چکیده
منابع مشابه
A robust TALENs system for highly efficient mammalian genome editing
Recently, transcription activator-like effector nucleases (TALENs) have emerged as a highly effective tool for genomic editing. A pair of TALENs binds to two DNA recognition sites separated by a spacer sequence, and the dimerized FokI nucleases at the C terminal then cleave DNA in the spacer. Because of its modular design and capacity to precisely target almost any desired genomic locus, TALEN ...
متن کاملUltrasonic-assisted Method for Preparation of Cu-doped ZnS Nanoparticles in Water as a Highly Efficient Visible Light Photocatalyst
Ultrasonic-assisted method was applied for preparation of Cu+2-doped ZnS nanoparticles (mole fractions of Cu+2 ions are 0.000, 0.015, 0.030 and 0.060) in water as a template-free and green method at 30 min. The prepared nanoparticles were investigated by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and UV–Vis diffuse reflectance spectra (DRS)...
متن کاملChemical Dissection of Mammalian Spermatozoa
Mammalian spermatozoa have been dissected by a variety of chemical techniques to yield free heads, tails with attached midpieces, and tails without mitochondria. By brief exposure to trypsin, mouse and rat spermatozoa were cleaved at the junction of the head and the tail, while human, guinea pig and rabbit spermatozoa were cleaved by trypsin only after prior incubation with a sulphhydryl reduci...
متن کاملA Simple, Highly Efficient Method for Heterologous Expression in Mammalian Primary Neurons Using Cationic Lipid-mediated mRNA Transfection
Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The post-mitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods. Adequate heterologous protein expression is often only achievable using complex techniques that, in many cases, are associated with substantial toxicity...
متن کاملHighly efficient catalyst for removal of heavy metal ions modified by a novel Schiff base ligand
An alomina-based nano adsorbent was prepared by modification of the external surface of γ-alumina (γ-Al2O3) nanoparticles with functional groups of a new Schiff base 4-[(2-hydroxy-3-methoxy-benzylidene)-amino]-5-methyl-2,4-dihydro-[1,2.4]triazole-3-thione] “L”. In order to the removal of Cr(VI) from aqueous solutions, we used the reaction of the sodium dodecyl sulfate coated nano-alumina with L...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction
سال: 1976
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0480347